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STEMCELL Technologies Inc brainphys tm neuronal medium
Brainphys Tm Neuronal Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brainphys+tm+neuronal+medium/brainphys+neuronal+medium/pmc12001802-244-14-18
Average 90 stars, based on 1 article reviews
brainphys tm neuronal medium - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Concentration Assay:

Article Title: Comparison of Microglial Morphology and Function in Primary Cerebellar Cell Cultures on Collagen and Collagen-Mimetic Hydrogels
Article Snippet: .. Briefly, the neuronal-glial cells on day 7 in culture were loaded with the cell-permeable Ca 2+ -sensitive dye Oregon Green TM 488 BAPTA-1, AM (OGB-AM, Thermo Fisher Scientific) in BrainPhys TM Neuronal Medium (Stemcell Technologies, Vancouver, BC, Canada) for 15 min. BrainPhys TM Neuronal Medium was used for washing extracellular dye and recording Ca 2+ concentration. .. Images were acquired and analyzed with the Solis software (Andor Technology Ltd., Belfast, UK); ImageJ and custom-written algorithms were also used for analysis.

Purification:

Article Title: Sphingomyelinases in retinas and optic nerve heads: Effects of ocular hypertension and ischemia
Article Snippet: The microbead-containing cells were enriched on a MACS MS column (Miltenyi Biotec, Germany) following the manufacturer’s instructions. .. Purified cells were cultured overnight in BrainPhys TM Neuronal medium (Stem Cell Technologies, Canada) including B27 supplement (Thermo Fisher Scientific, MA) on matrigel (Corning, AZ) coated slides. ..

Cell Culture:

Article Title: Sphingomyelinases in retinas and optic nerve heads: Effects of ocular hypertension and ischemia
Article Snippet: The microbead-containing cells were enriched on a MACS MS column (Miltenyi Biotec, Germany) following the manufacturer’s instructions. .. Purified cells were cultured overnight in BrainPhys TM Neuronal medium (Stem Cell Technologies, Canada) including B27 supplement (Thermo Fisher Scientific, MA) on matrigel (Corning, AZ) coated slides. ..

Article Title: Schwann Cells in the Aganglionic Colon of Hirschsprung Disease Can Generate Neurons for Regenerative Therapy
Article Snippet: Images were taken using a Nikon A1R laser scanning confocal microscope (Nikon Instruments, Melville, NY) or a Keyence BZX-700 All-In-One Microscopy (Keyence America Itasca, IL). .. For imaging of in vitro cultured GCaMP5 cells, NLBs grown from Wnt1-G5-tdT HSCR mice were transferred to fibronectin-coated (1:500 of sterile PBS for 1h at 37 °C) cell culture plates and grown in BrainPhys TM neuronal medium (STEMCELL Technologies) supplemented with N2 (1%, STEMCELL Technologies) and SM1 (2%, STEMCELL Technologies), for 7 days. ..

Article Title: A novel neurodevelopmental syndrome caused by loss-of-function of the Zinc Finger Homeobox 3 (ZFHX3) gene
Article Snippet: .. Neural stem cells were cultured in BrainPhys TM Neuronal Medium (STEMCELL Technologies), supplemented with NeuroCultTM SM1 Neuronal Supplement (STEMCELL Technologies), N2 Supplement-A (StemCell Technologies), recombinant Human Brain Derived Neurotrophic Factor (BDNF, PeproTech, 20 ng/mL), recombinant Human Glial-Derived Neurotrophic Factor (GDNF, PeproTech, 20 ng/mL), dibutyryl cAMP (1 mM, Sigma), and ascorbic acid (200 nM, Sigma). ..

Article Title: Phosphorylated-tau associates with HSV-1 chromatin and correlates with nuclear speckles decondensation in low-density host chromatin regions
Article Snippet: .. After 2 h, the inocula were removed, the organoids were washed and cultured in BrainPhys TM Neuronal medium (StemCell Technologies). .. The following HSV-1 strains were employed in this study: KOS (VR-1493; ATCC); 17 syn +, McIntyre, and McKrae (gifts from Dr. D. Bloom from stocks obtained from Dr. J. Stevens).

Imaging:

Article Title: Schwann Cells in the Aganglionic Colon of Hirschsprung Disease Can Generate Neurons for Regenerative Therapy
Article Snippet: Images were taken using a Nikon A1R laser scanning confocal microscope (Nikon Instruments, Melville, NY) or a Keyence BZX-700 All-In-One Microscopy (Keyence America Itasca, IL). .. For imaging of in vitro cultured GCaMP5 cells, NLBs grown from Wnt1-G5-tdT HSCR mice were transferred to fibronectin-coated (1:500 of sterile PBS for 1h at 37 °C) cell culture plates and grown in BrainPhys TM neuronal medium (STEMCELL Technologies) supplemented with N2 (1%, STEMCELL Technologies) and SM1 (2%, STEMCELL Technologies), for 7 days. ..

In Vitro:

Article Title: Schwann Cells in the Aganglionic Colon of Hirschsprung Disease Can Generate Neurons for Regenerative Therapy
Article Snippet: Images were taken using a Nikon A1R laser scanning confocal microscope (Nikon Instruments, Melville, NY) or a Keyence BZX-700 All-In-One Microscopy (Keyence America Itasca, IL). .. For imaging of in vitro cultured GCaMP5 cells, NLBs grown from Wnt1-G5-tdT HSCR mice were transferred to fibronectin-coated (1:500 of sterile PBS for 1h at 37 °C) cell culture plates and grown in BrainPhys TM neuronal medium (STEMCELL Technologies) supplemented with N2 (1%, STEMCELL Technologies) and SM1 (2%, STEMCELL Technologies), for 7 days. ..

Sterility:

Article Title: Schwann Cells in the Aganglionic Colon of Hirschsprung Disease Can Generate Neurons for Regenerative Therapy
Article Snippet: Images were taken using a Nikon A1R laser scanning confocal microscope (Nikon Instruments, Melville, NY) or a Keyence BZX-700 All-In-One Microscopy (Keyence America Itasca, IL). .. For imaging of in vitro cultured GCaMP5 cells, NLBs grown from Wnt1-G5-tdT HSCR mice were transferred to fibronectin-coated (1:500 of sterile PBS for 1h at 37 °C) cell culture plates and grown in BrainPhys TM neuronal medium (STEMCELL Technologies) supplemented with N2 (1%, STEMCELL Technologies) and SM1 (2%, STEMCELL Technologies), for 7 days. ..

Recombinant:

Article Title: A novel neurodevelopmental syndrome caused by loss-of-function of the Zinc Finger Homeobox 3 (ZFHX3) gene
Article Snippet: .. Neural stem cells were cultured in BrainPhys TM Neuronal Medium (STEMCELL Technologies), supplemented with NeuroCultTM SM1 Neuronal Supplement (STEMCELL Technologies), N2 Supplement-A (StemCell Technologies), recombinant Human Brain Derived Neurotrophic Factor (BDNF, PeproTech, 20 ng/mL), recombinant Human Glial-Derived Neurotrophic Factor (GDNF, PeproTech, 20 ng/mL), dibutyryl cAMP (1 mM, Sigma), and ascorbic acid (200 nM, Sigma). ..

Article Title: Activated microglia and neuroinflammation as a pathogenic mechanism in Leigh syndrome
Article Snippet: .. Neuronal precursors were seeded at a density of 3 * 10 4 cells/cm 2 and neurons were allowed to mature for at least 1 week in STEMdiff TM Neuron Maturation medium or Brainphys TM Neuronal medium (STEMCELL, catalog #05790) supplemented with Neurocult TM SM1 Neuronal supplement (STEMCELL, catalog #05711), N2 supplement-A (STEMCELL, catalog #07152), recombinant human brain derived neurotrophic factor, recombinant human glial-derived neurotrophic factor, dibutyryl cAMP, and ascorbic acid. ..

Derivative Assay:

Article Title: A novel neurodevelopmental syndrome caused by loss-of-function of the Zinc Finger Homeobox 3 (ZFHX3) gene
Article Snippet: .. Neural stem cells were cultured in BrainPhys TM Neuronal Medium (STEMCELL Technologies), supplemented with NeuroCultTM SM1 Neuronal Supplement (STEMCELL Technologies), N2 Supplement-A (StemCell Technologies), recombinant Human Brain Derived Neurotrophic Factor (BDNF, PeproTech, 20 ng/mL), recombinant Human Glial-Derived Neurotrophic Factor (GDNF, PeproTech, 20 ng/mL), dibutyryl cAMP (1 mM, Sigma), and ascorbic acid (200 nM, Sigma). ..

Article Title: Activated microglia and neuroinflammation as a pathogenic mechanism in Leigh syndrome
Article Snippet: .. Neuronal precursors were seeded at a density of 3 * 10 4 cells/cm 2 and neurons were allowed to mature for at least 1 week in STEMdiff TM Neuron Maturation medium or Brainphys TM Neuronal medium (STEMCELL, catalog #05790) supplemented with Neurocult TM SM1 Neuronal supplement (STEMCELL, catalog #05711), N2 supplement-A (STEMCELL, catalog #07152), recombinant human brain derived neurotrophic factor, recombinant human glial-derived neurotrophic factor, dibutyryl cAMP, and ascorbic acid. ..

other:

Article Title: Loss of Non-motor Kinesin KIF26A Causes Congenital Brain Malformations via Dysregulated Neuronal Migration and Axonal Growth as well as Apoptosis
Article Snippet: BrainPhys TM Neuronal Medium and SM1 Kit , StemCell Technologies , cat. # 05792.

Microelectrode Array:

Article Title: CRISPRi-based screens in iAssembloids to elucidate neuron-glia interactions
Article Snippet: Sequencing parameters and quality control were performed as described by The Tabula Muris Consortium . .. 6 (Axion Biosystems, Cat. No. M384-tMEA-6B and 24 (Axion Biosystems, Cat. No. M384-tMEA-24W) well Cytoview MEA plates were coated with poly-L-ornithine (0.01%), fibronectin (1 μg/mL) and laminin (5 μg/mL) to facilitate iAssembloid adherence. iAssembloids were positioned into the well over the electrodes using a microscope. iAssembloids were equilibrated in BrainPhys TM Neuronal Medium (StemCell Technologies, Cat. No. 05790) 24 hours before plating to facilitate comparison to 2D monocultured neurons. .. Measurements were taken using the Maestro Edge multiwell microelectrode array (MEA) and Impedance system (Axion Biosystems) at 15 minute intervals with the Maestro MEA platform software (Axion Biosystems) with the “Neural” default setting.

Microscopy:

Article Title: CRISPRi-based screens in iAssembloids to elucidate neuron-glia interactions
Article Snippet: Sequencing parameters and quality control were performed as described by The Tabula Muris Consortium . .. 6 (Axion Biosystems, Cat. No. M384-tMEA-6B and 24 (Axion Biosystems, Cat. No. M384-tMEA-24W) well Cytoview MEA plates were coated with poly-L-ornithine (0.01%), fibronectin (1 μg/mL) and laminin (5 μg/mL) to facilitate iAssembloid adherence. iAssembloids were positioned into the well over the electrodes using a microscope. iAssembloids were equilibrated in BrainPhys TM Neuronal Medium (StemCell Technologies, Cat. No. 05790) 24 hours before plating to facilitate comparison to 2D monocultured neurons. .. Measurements were taken using the Maestro Edge multiwell microelectrode array (MEA) and Impedance system (Axion Biosystems) at 15 minute intervals with the Maestro MEA platform software (Axion Biosystems) with the “Neural” default setting.

Comparison:

Article Title: CRISPRi-based screens in iAssembloids to elucidate neuron-glia interactions
Article Snippet: Sequencing parameters and quality control were performed as described by The Tabula Muris Consortium . .. 6 (Axion Biosystems, Cat. No. M384-tMEA-6B and 24 (Axion Biosystems, Cat. No. M384-tMEA-24W) well Cytoview MEA plates were coated with poly-L-ornithine (0.01%), fibronectin (1 μg/mL) and laminin (5 μg/mL) to facilitate iAssembloid adherence. iAssembloids were positioned into the well over the electrodes using a microscope. iAssembloids were equilibrated in BrainPhys TM Neuronal Medium (StemCell Technologies, Cat. No. 05790) 24 hours before plating to facilitate comparison to 2D monocultured neurons. .. Measurements were taken using the Maestro Edge multiwell microelectrode array (MEA) and Impedance system (Axion Biosystems) at 15 minute intervals with the Maestro MEA platform software (Axion Biosystems) with the “Neural” default setting.



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